RAFAH, Gaza Strip >> Hamas today released two elderly Israeli women held hostage in Gaza as the United States expressed increasing concern that the escalating Israel-Hamas war will spark a wider conflict in the region, including attacks on American troops.

The death toll in Gaza rose rapidly as Israel ramped up airstrikes that flattened buildings in what it said was preparation for an eventual ground assault. The United States advised Israel to delay the expected invasion to allow time to negotiate the release of more hostages taken by Hamas during its brutal incursion two weeks ago.

A third small aid convoy from Egypt entered Gaza, where the population of 2.3 million has been running out of food, water and medicine under Israel’s sealed border. With Israel still barring entry of fuel, the U.N. said its distribution of aid would grind to a halt within days when it can no longer fuel its trucks. Gaza hospitals flooded by a constant stream of wounded are struggling to keep generators running to power lifesaving medical equipment and incubators for premature babies.

The two freed hostages, 85-year-old Yocheved Lifshitz and 79-year-old Nurit Cooper, were taken out of Gaza at the Rafah crossing into Egypt, where they were put into ambulances, according to footage shown on Egyptian TV. The two women, along with their husbands, were snatched from their homes in the kibbutz of Nir Oz near the Gaza border during Hamas’ Oct. 7 rampage into southern Israeli communities. Their husbands, ages 83 and 84, were not released.

“While I cannot put into words the relief that she is now safe, I will remain focused on securing the release of my father and all those — some 200 innocent people — who remain hostages in Gaza,” Lifshitz’ daughter, Sharone Lifschitz, said in a statement.

Lifschitz, an artist and academic in London who uses a different spelling for her name, told reporters last week that her parents were peace activists, and her father would drive to the Gaza border to take Palestinians to east Jerusalem for medical treatment.

Kindness, she said last week, could somehow save them.

“I grew up, you know, with all these Holocaust stories about how all my uncles’ lives were saved because” of acts of kindness, she said.

“Do I want that to be the story here?” she asked. “Yeah.”

Hamas apparently received nothing in exchange for the release of the two hostages, who were freed days after an American woman and her teenage daughter. Hamas and other militants in Gaza are believed to have taken roughly 220 people, including an unconfirmed number of foreigners and dual citizens.

Israel has vowed to destroy Hamas. Iranian-backed fighters around the region are warning of possible escalation, including the targeting of U.S. forces deployed in the Mideast, if a ground offensive is launched in Gaza.

The U.S. has told Iranian-backed Hezbollah in Lebanon and other groups not to join the fight. Israel and Hezbollah have traded fire almost daily across the Israel-Lebanon border, and Israeli warplanes have struck targets in the occupied West Bank, Syria and Lebanon in recent days.

National Security Council spokesman John Kirby said there has been an uptick in rocket and drone attacks by Iranian-backed militias on U.S. troops in Iraq and Syria, and the U.S. was “deeply concerned about the possibility for any significant escalation” in the coming days.

He said U.S. officials were having “active conversations” with Israeli counterparts about the potential ramifications of escalated military action.

The U.S. advised Israeli officials that delaying a ground offensive would give Washington more time to work with regional mediators on the release of more hostages, according to a U.S. official.

Israeli tanks and ground forces have been massed at the Gaza border, and Defense Minister Yoav Gallant told troops there Monday to keep preparing for an offensive “because it will come.” He said it will be a combined offensive from air, land and sea, but he did not give a time frame.

A ground offensive is likely to dramatically increase casualties in what is already the deadliest by far of five wars fought between Israel and Hamas since the militant group took power in Gaza in 2007.

More than 1,400 people in Israel have been killed — mostly civilians slain during the initial Hamas attack. At least 222 people were captured and dragged back to Gaza, including foreigners, the military said Monday, updating a previous figure.

More than 5,000 Palestinians, including some 2,000 minors and around 1,100 women, have been killed, the Hamas-run Health Ministry said Monday. That includes the disputed toll from an explosion at a hospital last week. The toll has climbed rapidly in recent days, with the ministry reporting 436 additional deaths in just the last 24 hours.

Israel said it struck 320 militant targets throughout Gaza over the last 24 hours. The military says it does not target civilians, and that Palestinian militants have fired over 7,000 rockets at Israel since the start of the war.

Inside Gaza, the civilian death toll continued to mount.

Fifteen members of the same family were among at least 33 Palestinians buried Monday in a shallow, sandy mass grave at a Gaza hospital after being killed in Israeli airstrikes.

The bodies were laid to rest side by side in the courtyard of al-Aqsa Hospital in Deir al-Balah. Men discussed where to fit the shrouded corpse of a small child. “Bring them all,” a gravedigger called out.

Israel carried out limited ground forays into Gaza. On Sunday, Hamas said it destroyed an Israeli tank and two armored bulldozers inside Gaza. The Israeli military said a soldier was killed and three others were wounded by an anti-tank missile during a raid inside Gaza.

On Monday, the Palestinian Red Crescent said 20 trucks entered Gaza carrying food, water, medicine and medical supplies through the Rafah crossing with Egypt, the only way into Gaza not controlled by Israel. It was the third delivery in as many days, each around the same size.

The aid coming in so far is “a drop in the ocean” compared with the needs of the population, said Thomas White, the Gaza director of the U.N. agency for Palestinian refugees, UNRWA. The U.N. has said 20 trucks amounts to 4% of an average day’s imports before the war and that hundreds of trucks a day are needed.

White said the agency had only three days of fuel left for its trucks. The supplies coming through Rafah are reloaded onto UNRWA vehicles and the Red Crescent trucks to take to hospitals and U.N. schools in the south of Gaza, where hundreds of thousands of people are taking shelter, running low on food and largely drinking contaminated water.

At least 1.4 million Palestinians in Gaza have fled their homes, and nearly 580,000 of them are sheltering in U.N.-run schools and shelters, the U.N. said Monday.

No aid will be distributed in Gaza City and other parts of the north, where hundreds of thousands of people remain. Gaza City’s main al-Shifa Hospital, with a normal capacity of 700 patients, is currently overwhelmed with 5,000 patients, and around 45,000 displaced people are gathered in and around its grounds for shelter, the U.N. said.

Magdy reported from Cairo and Krauss from Jerusalem. Associated Press writers Wafaa Shurafa in Deir al-Balah, Gaza Strip, Aamer Madhani in Washington, Amy Teibel in Jerusalem, Brian Melley in London, contributed to this report.